So I'm working on identifying the phosphorylation sites of my protein, Xp95. I got it narrowed down to a roughly 100 amino acid region, which contains about 15 possible phosphorylation sites, about half of which are conserved (and more likely to be phosphorylated). I finished a mutation screen, making four new mutants. All four are still phosphorylated, which is pretty bad for me, i was 99% sure I'd find it in one of the four. I have 2 conserved and 3 non-conserved sites left to examine. Since this is the entire thrust of my thesis I now wonder if/when I will graduate. Certainly not good news.